OMAX - FBA_CS-A36CALM1 0.01mm Microscope Camera Calibration Slide (Stage Micrometer)

Seller Amazon does themselves a disservice by not showing a clearer image of the calibration marks on this slide. The marks are photo-lithographed metal, probably sputtered onto the slide in a vacuum chamber similar to a semiconductor manufacturing process. The circle surrounding the 1.0 mm ruled line is about 3 mm in diameter and approximately 30-45 microns wide. The 1.0 mm ruled line is divided into major divisions of tenths, or 100 microns per major division. The major divisions are delineated by longest lines. Each major division is divided into two intermediate divisions by intermediate-length lines; so 50 microns per intermediate division. Each intermediate division is divided into five smallest divisions of 10 microns each. I estimate the line width delineating each small division to be about 2 microns wide; so were talking about very precision manufacturing for this calibration slide. Note that I cannot vouch for the absolute accuracy of the length of the full 1.0 mm graduated line. I can simply report that the full graduated line is divided very evenly and precisely into 100 small (~10 micron) divisions. Very impressed. The only negative that I have to report is that the ridges inside the plastic cover do not support the slide thickness very precisely. Consequently the slide has some play within the case when transported. Wrapping the slide back up in the thin paper in which it is originally packaged inside the plastic storage case solves that problem.

The ruler is a thick film metalization layer on the glass. This makes the quality much higher than the optically exposed film slide that I broke and had to replace! Nice!

Great slide and excellent image. Even better price for such an important tool to calibrate your microscope reticle or IA software.

Works well for calibration.

just......wow!

I was wondering is there away by looking how many lines I can see what kind of magnification I have? I can count 8 lines using a 40 power objective with 10 power ocular. Thanks

just what it should be.

It was quite difficult to locate the small target zone under field conditions, but the markings are sharp. I cant vouch for the calibration without having compared against an expensive standard, but it matched almost exactly against another similar product.

I used this reticle to directly check the magnification of a digital microscope. The image of the reticle can be directly measure on the screen. (I found that the advertisement of the microscope in question contained an exaggeration.) With this information, the sizes of objects being viewed can be calculated directly. Of course, magnification is only half of the practical question -- the other half being resolution. With the clean lines on the reticle, one can get an idea of that too, but seeing by how many pixels the sharp line edges are blurred on the screen.

Meets specs exactly and I got it fast. Good job.